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ID 109766
タイトルヨミ
コツガ サイボウ ノ ブンカ ニオケル PKR ノ ヤクワリ ニツイテ
タイトル別表記
Roles of Double-stranded RNA-dependent Protein Kinase on the Differentiation of Osteoblasts
著者
吉田, 賀弥 徳島大学歯学部口腔保健学科口腔保健基礎学講座 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
PKR
osteoblast
Runx2
STAT1
資料タイプ
学術雑誌論文
抄録
In this study, it was demonstrated that double-stranded RNA-dependent protein kinase (PKR) is required for the differentiation of osteoblasts. PKR was expressed on the surface of trabecula in rat tibiae. Dominant-negative mutant PKR cDNA, in which the amino acid lysine at 296 was replaced with arginine and which did not have catalytic activity, was transfected into mouse osteoblastic MC3T3-E1 cells; thereby establishing cells that stably expressed the PKR mutant gene (PKR-K/R). PKR-K/R mutant cells exhibited up-regulated cell growth and had low alkaline phosphatase (ALP) activity. PKR-K/R mutant cells were not able to form bone nodules in vitro. The expression of STAT1α protein increased in PKR-K/R mutant cells, in which runt-related gene 2 (Runx2)-mediated transcription decreased compared with the levels in control cells. When the expression of STAT1α protein in PKR mutant cells was suppressed in the presence of STAT1α siRNA, the activity of Runx2-mediated transcription recovered to the control level. These results indicate that PKR is a stimulator of Runx2 transcription and is a negative modulator of STAT1α expression. These findings also suggest that PKR plays important roles in the differentiation and calcification of osteoblasts by modulating STAT1α and/or Runx2 expression.
掲載誌名
四国歯学会雑誌
ISSN
09146091
cat書誌ID
AN10050046
出版者
四国歯学会
20
2
開始ページ
223
終了ページ
227
並び順
223
発行日
2008-01-31
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
歯学系