ID 109875
タイトルヨミ
シニクコウ シンシュツエキ チュウ ノ バイオマーカー オ モチイタ シシュウビョウ シンダン
タイトル別表記
Diagnosis of Periodontal Diseases using Biomarkers in Gingival Crevicular Fluid
著者
木戸, 淳一 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
板東, 美香 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
稲垣, 裕司 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
生田, 貴久 徳島大学大学院医歯薬学研究部歯周歯内治療学分野
梶浦, 由加里 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 KAKEN研究者をさがす
中島, 由紀子 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
坂本, 英次郎 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧
美原, 智恵 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
二宮, 雅美 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
西川, 泰史 徳島大学大学院医歯薬学研究部歯周歯内治療学分野
Lew, Jung-Hwan 徳島大学大学院医歯薬学研究部歯周歯内治療学分野
成石, 浩司 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
永田, 俊彦 徳島大学大学院医歯薬学研究部歯周歯内治療学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
歯周病診断
バイオマーカー
歯肉溝滲出液
資料タイプ
学術雑誌論文
抄録
Periodontal diseases cause an inflammation and degradation of periodontal tissues and missing of teeth. The incidence rate of periodontal diseases is high in middle-aged and elderly people.
A reasonable diagnosis of periodontal diseases is very important to keep teeth, however, conventional examinations of periodontal diseases is not necessarily exact and objective. Gingival crevicular fluid (GCF) is an exudate secreted from periodontal tissues and contains many components including proteolytic enzymes, inflammatory cytokines, blood-associated proteins, cellular and bacterial fragments. Because some proteins in GCF are related to inflammation, tissue degradation and bone metabolism, those proteins have been studying as a diagnostic marker of periodontal diseases. GCF is noninvasively collected using a sterile paper strip and biomarkers are determined using enzyme-linked immunosorbent assay (ELISA) and enzyme activity assay. We identified calprotectin, an inflammationrelated protein, in GCF and calprotectin level in GCF from periodontitis sites was significantly higher than that of healthy control. Calprotectin level in GCF was positively correlated to gingival index and other biomarkers and decreased by periodontal treatments. Resistin is an adipocytokine and its level increases in some inflammatory diseases. Resistin level in GCF from periodontitis sites was high compared to the level of healthy control samples. Procollagen type I C-terminal peptide (PICP) is a biomarker for bone metabolism and its level was high in GCF collected from periodontitis sites. These results suggested that calprotectin, resistin and PICP are useful biomarkers for periodontal diseases. On the other hand, we showed that glycated albumin (GA), a marker of diabetes mellitus (DM), was contained in GCF and GA level in GCF from DM patients was significantly higher than that of non-DM individuals.
Components in GCF may be biomarkers of systemic diseases as well as periodontal diseases and their determination will be useful diagnostic examination of some diseases. Recently, we have been studying the determining system of GCF calprotectin, including microchip ELISA, surface plasmon resonance assay and immuno-chromatography assay. When GCF biomarkers are determined using the determining systems, we will simply, exactly and objectively diagnose periodontal diseases at our dental offices.
掲載誌名
Journal of Oral Health and Biosciences
ISSN
21887888
cat書誌ID
AA12713630
出版者
四国歯学会
29
1
開始ページ
13
終了ページ
20
並び順
13
発行日
2016-06-30
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
歯学系
病院