アクセス数 : ?
ダウンロード数 : ?
ID 110107
タイトル別表記
DNAメチル化は3T3-L1脂肪細胞におけるレプチン発現を抑制する
DNA Methylation Suppresses Leptin Expression in Adipocytes
著者
黒田, 雅士 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻) 徳島大学 教育研究者総覧
Tominaga, Ayako Tokushima University
Nakagawa, Kasumi Tokushima University
Nishiguchi, Misa Tokushima University
Sebe, Mayu Tokushima University
Miyatake, Yumiko Tokushima University
Kitamura, Tadahiro Japan Society for the Promotion of Science
キーワード
Adipocyte
Leptin
DNA methylation
3T3-L1
obesity
資料タイプ
学位論文
抄録
Leptin is a key regulator of energy intake and expenditure. This peptide hormone is expressed in mouse white adipose tissue, but hardly expressed in 3T3-L1 adipocytes. Using bisulfite sequencing, we found that CpG islands in the leptin promoter are highly methylated in 3T3-L1cells. 5-azacytidine, an inhibitor of DNA methyltransferase, markedly increased leptin expression as pre-adipocytes matured into adipocytes. Remarkably, leptin expression was stimulated by insulin in adipocytes derived from precursor cells exposed to 5-azacytidine, but suppressed by thiazolidinedione and dexamethasone. In contrast, adipocytes derived from untreated precursor cells were unresponsive to both 5-azacytidine and hormonal stimuli, although lipid accumulation was sufficient to boost leptin expression in the absence of demethylation. Taken together, the results suggest that leptin expression in 3T3-L1 cells requires DNA demethylation prior to adipogenesis, transcriptional activation during adipogenesis, and lipid accumulation after adipogenesis.
掲載誌名
PLOS ONE
ISSN
19326203
出版者
PLOS
11
8
開始ページ
e0160532
発行日
2016-08-05
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704261022.pdf
論文本文:LID201704261023.pdf
本論文は, 著者Masashi Kurodaの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3087号
学位記番号
甲栄第239号
学位授与年月日
2017-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系