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ID 110108
タイトル別表記
Campylobacter jejuni感染はT-84細胞におけるCFTR活性化によるCl⁻分泌亢進を抑制する
著者
天野, 幸恵 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Hatayama, Syo The University of Tokushima
Sato, Yuri The University of Tokushima
Matsumoto, Mari The University of Tokushima
Iba, Hitomi The University of Tokushima
Nishikawa, Yoshikazu Osaka City University
Yamasaki, Shinji Osaka Prefecture University
キーワード
Campylobacter jejuni
CFTR
diarrhea
short-circuit current
efflux assay
資料タイプ
学位論文
抄録
Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl-) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl- channel transporting Cl- to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied.
Here, we used an 125I- efflux assay and measurement of short-circuit current to measure Cl- secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl- secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl- secretion suppressed in C. jejuniinfected T-84 cells. The suppression of activated Cl- secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract.
掲載誌名
Journal of Infection and Chemotherapy
ISSN
1341321X
cat書誌ID
AA11057978
AA11627318
出版者
Japanese Society of Chemotherapy and The Japanese Association for Infection Disease|Elsevier
20
11
開始ページ
682
終了ページ
688
発行日
2014-08-06
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201704261024.pdf
論文本文:LID201704261025.pdf
本論文は, 著者Sachie Negoroの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3089号
学位記番号
甲栄第241号
学位授与年月日
2017-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系
生物資源系