ID 110160
著者
イトウ, タカノリ Department of Gastroenterology and Hepatology
イシガミ, マサトシ Department of Gastroenterology and Hepatology
マツシタ, ヨシヒロ Department of Oral and Maxillofacial Surgery of Nagoya University Graduate School of Medicine
ヒラタ, マリナ Department of Oral and Maxillofacial Surgery of Nagoya University Graduate School of Medicine
マツバラ, コウキ Department of Oral and Maxillofacial Surgery of Nagoya University Graduate School of Medicine
イシカワ, テツヤ Department of Gastroenterology and Hepatology
ヒビ, ヒデハル Department of Oral and Maxillofacial Surgery of Nagoya University Graduate School of Medicine
ウエダ, ミノル Department of Oral and Maxillofacial Surgery of Nagoya University Graduate School of Medicine
ヒロオカ, ヨシキ Department of Gastroenterology and Hepatology
ゴトウ, ヒデミ Department of Gastroenterology and Hepatology
山本, 朗仁 Department of Oral and Maxillofacial Surgery of Nagoya University Graduate School of Medicine|Department of Oral histology, Institute of Biomedical Science, Tokushima University Graduate School 徳島大学 教育研究者総覧
資料タイプ
学術雑誌論文
抄録
Effective treatments for acute liver failure (ALF) are still lacking. We recently reported that a single intravenous administration of serum-free conditioned medium from stem cells derived from human exfoliated deciduous teeth (SHED-CM) into the D-galactosamine (D-Gal)-induced rat ALF model improves the liver injury. However, the specific factors in SHED-CM that are responsible for resolving ALF remain unclear. Here we found that depleting SHED-CM of two anti-inflammatory M2 macrophage inducers—monocyte chemoattractant protein-1 (MCP-1) and the secreted ectodomain of sialic acidbinding Ig-like lectin-9 (sSiglec-9)—abolished its ability to resolve rat ALF. Furthermore, treatment with MCP-1/sSiglec-9 alone dramatically improved the survival of ALF rats. This treatment induced anti-inflammatory M2, suppressed hepatocyte apoptosis, and promoted hepatocyte proliferation. Treatment with an M2-depletion reagent (mannosylated clodronate liposomes) suppressed the recovery. In addition, MCP-1 and sSiglec-9 synergistically promoted the M2 differentiation of bone marrow-derived macrophages via CCR2, accompanied by the production of multiple liver-regenerating factors. The conditioned medium from MCP-1/sSiglec-9-activated M2 macrophages, but not from interleukin-4-induced ones, suppressed the D-Gal- and LPS-induced apoptosis of primary hepatocytes and promoted their proliferation in vitro. The unique combination of MCP-1/sSiglec-9 ameliorates rat ALF by inhibiting hepatocellular apoptosis and promoting liver regeneration through the induction of anti-inflammatory/tissue-repairing M2 macrophages.
掲載誌名
Scientific Reports
ISSN
20452322
7
開始ページ
44043
並び順
44043
発行日
2017-03-08
備考
© The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系