ID 110638
著者
Momen, Mohammed Abdul Second Department of Surgery, University Hospital, The University of Tokushima School of Medicine
門田, 康正 Second Department of Surgery, University Hospital, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
ホウチ, ヒトシ Division of Pharmacy, University Hospital, The University of Tokushima School of Medicine
ウメモト, アツシ Second Department of Surgery, University Hospital, The University of Tokushima School of Medicine
キーワード
ursodeoxycholic acid
aberrant crypt foci
colon cancer
bile acid
intracellular Ca2+
資料タイプ
学術雑誌論文
抄録
The studies were conducted to examine the precise nature of the suppressive effect of ursodeoxycholic acid (UDCA) on colonic aberrant crypt foci (ACF) formation. Fischer 344 rats were treated with a single dose of azoxymethane (AOM) (20 mg/kg, s.c.) and fed basal diet (MF) supplemented with UDCA (0.4%) during an initiation or a post-initiation stage. ACF were enumerated at the 2nd, 5th and 8th weeks after AOM administration (15-18 rats/group).The number of ACF in the UDCA treated group was decreased significantly in the initiation and post-initiation stages at the 2nd (Plt0.01, Plt0.0001) and 8th weeks (Plt0.001, Plt0.0001),respectively, compared with untreated controls. In the time-course experiments, the effect of continuous feeding of UDCA (0.4%) on ACF formation was evaluated. ACF number was decreased significantly (Plt0.005) until the 16thweek.UDCAshowed a significant dose-dependent suppression of ACF number from a range of 0.1-0.4%UDCA.To approach the subcellular mechanisms of the effect of bile acids, the intracellular free Ca2+ concentration ([Ca2+]i) of bile acid-treated rat colonic cancer cells (ACL-15) was examined. DCA and CDCA, which are promotive on ACF formation, induced a rapid increase in [Ca2+]i, while UDCA and CA, which are suppressive or non-effective on ACF formation, did not. These findings suggest that the promotive effect of bile acids may involve intracellular Ca2+ signaling.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
49
1-2
開始ページ
67
終了ページ
73
並び順
67
発行日
2002
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系