ID 110639
著者
小川, 博久 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ニシムラ, ナオキ Third Department of Internal Medicine, The University of Tokushima School of Medicine
西岡, 安彦 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
吾妻, 雅彦 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
楊河, 宏章 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
曽根, 三郎 Third Department of Internal Medicine, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
IL-12
BEAS-2B
adenoviral vector
資料タイプ
学術雑誌論文
抄録
Interleukin (IL)-12 is known as a cytokine that augments the Th1 type response. Especially in allergic diseases such as a bronchial asthma, IL-12 induced restoration of the balance of the Th1/Th2 type immune response is an attractive strategy. In this study, the functional properties of the human bronchial epithelial cell line (BEAS-2B) transduced by an adenoviral vector encoding the human IL-12 gene were examined. Adenovirus vectors, AxCAegfp and Ax1CIhp40ip35 were transduced into BEAS -2B cells. Wild and gene-transduced BEAS -2B cells were incubated and the concentrations of IL-12and IFN-γ produced by co-cultured lymphocytes in the supernatant were measured using ELISA. The expressions of surface adhesion molecules, such as CD54 and CD106 were analyzed using flow cytometry. The efficiency of transgene expression of BEAS-2B cells was in a multiplicity of infection(MOI)-dependent manner and at an MOI of 30, the efficiency was approximately 80%. The gene-modified BEAS-2B cells produced biologically active IL-12 in dose - and time-dependent manners. IL-12 gene transduction did not significantly affect the expression of adhesion molecules (CD 54, CD106 and HLA-A,B,C) by BEAS-2B cells. These results suggest that the IL-12 gene may be successfully transduced into human bronchial epithelial cells by adenoviral vector to express IL-12 activity in vivo.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
49
1-2
開始ページ
74
終了ページ
82
並び順
74
発行日
2002
EDB ID
146202
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系
病院