ID 110660
著者
ヨコイ, ヒロミチ Department of Anatomy and Cell Biology, The University of Tokushima School of Medicine
鶴尾, 吉宏 Department of Anatomy and Cell Biology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ニヒラ, ミカ Department of Anatomy and Cell Biology, The University of Tokushima School of Medicine
石村, 和敬 Department of Anatomy and Cell Biology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
17α-hydroxylase/C17
20-lyase
rat
placenta
immunocytochemistry
electron microscopy
資料タイプ
学術雑誌論文
抄録
The rat placenta is the primary source of androgens during the second half of pregnancy. Androgens are converted to estrogens in the ovaries and contribute to the maintenance of normal pregnancy. We immunocytochemically characterized the cellular and subcellular localization of cytochrome P450 of 17α-hydroxylase/C 17,20-lyase (P450c17), an enzyme responsible for androgen synthesis, in the rat placenta. We also observed the fine structure of the placenta by electron microscopy. The rat placenta had a different structure from the primate, and contained four zones :labyrinth, basal zone, decidua basalis, and metrial gland. The labyrinth had three trophoblastic layers and fetal endothelium, and P450c17 immunoreactivity was homogeneously localized in the three trophoblastic layers but not in the fetal endothelium. In the basal zone, various types of trophoblasts were observed, and the immunoreaction was localized in small basophilic cells and giant cells. The intensity of staining was heterogeneous among these cells. The decidua basalis showed no immunostaining. Subcellular localization of the enzyme was in the cytoplasm, but not in the nucleus or mitochondria. The present study demonstrated a steroidogenic potency in both the labyrinth and the basal zone, although it was shown only in the basal zone in previous studies.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
44
3-4
開始ページ
155
終了ページ
162
並び順
155
発行日
1998
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系