ID 110676
著者
カノウ, ミホコ Department of Nutrition, The University of Tokushima School of Medicine
キタノ, タカコ Department of Nutrition, The University of Tokushima School of Medicine
イケモト, マドカ Department of Nutrition, The University of Tokushima School of Medicine|Department of Neuromuscular Research, National Center of Neurology and Psychiatry
平坂, 勝也 Department of Nutrition, The University of Tokushima School of Medicine KAKEN研究者をさがす
アサノマ, ユキ Department of Nutrition, The University of Tokushima School of Medicine
オガワ, タカユキ Department of Orthopedic Surgery, The University of Tokushima School of Medicine
タケダ, シンイチ Department of Neuromuscular Research, National Center of Neurology and Psychiatry
ノナカ, イクヤ National Institute of Neuroscience, National Center of Neurology and Psychiatry
Adams, Gregory R Department of Physiology and Biophysics, University of California
Baldwin, Kenneth M Department of Physiology and Biophysics, University of California
オオアラダ, トモコ Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University
岸, 恭一 Department of Nutrition, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
二川, 健 Department of Nutrition, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
spaceflight
differential display approach
skeletal muscle gene
rats
資料タイプ
学術雑誌論文
抄録
We obtained the skeletal muscle of rats exposed to weightless conditions during a 16-day-spaceflight (STS-90). By using a differential display technique, we identified 6 up-regulated and 3 down-regulated genes in the gastrocnemius muscle of the spaceflight rats, as compared to the ground control. The up-regulated genes included those coding Casitas B-lineage lymphoma-b, insulin growth factor binding protein-1, titin and mitochondrial gene 16 S rRNA and two novel genes (function unknown). The down-regulated genes included those encoding RNA polymerase II elongation factor-like protein, NADH dehydrogenase and one novel gene (function unknown). In the present study, we isolated and characterize done of two novel muscle genes that were remarkably up-regulated by spaceflight. The deduced amino acid sequence of the spaceflight-induced gene (sfig) comprises 86amino acid residues and is well conserved from Drosophila to Homo sapiens. A putative leucine-zipper structure located at the N-terminal region of sfig suggests that this gene may encode a transcription factor. The up-regulated expression of this gene, confirmed by Northern blot analysis, was observed not only in the muscles of spaceflight rats but also in the muscles of tail-suspended rats, especially in the early stage of tail-suspension when gastrocnemius muscle atrophy initiated. The gene was predominantly expressed in the kidney, liver, small intestine and heart. When rat myoblastic L6 cells were grown to 100% confluence in the cell culture system, the expression of sfig was detected regardless of the cell differentiation state. These results suggest that spaceflight has many genetic effects on rat skeletal muscle.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
50
1-2
開始ページ
39
終了ページ
47
並び順
39
発行日
2003
EDB ID
146696
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系