ID 110763
著者
ミキ, ツヨシ Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
桑原, 知巳 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
中山, 治之 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
オカダ, ナツミ Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
片岡, 佳子 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
有持, 秀喜 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
大西, 克成 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧
キーワード
Bacteroides
leuB
PCR
rapid detection
資料タイプ
学術雑誌論文
抄録
Bacteroides species, saccharolytic Gram-negative obligate anaerobes, are frequently isolated from human infections such as peritonitis, abscesses and bacteremia. Among the species in the genus Bacteroides, thespecies called “B. fragilis group” areparticularly involved inhuman infections andaremedically important because they account for a major part of anaerobic isolates from clinical specimens. The purpose of this study was to develop PCR primers that specifically and simultaneously amplify theβ-isopropylmalate dehydrogenase gene leuB in B. fragilis group species. We determined partial nucleotide sequences of leuB genes and compared them in seventeen strains of nine B. fragilis group species, and the regions that are conserved among Bacteroides strains but different from other species were used as a B. fragilis group-specific PCR primer set, BacLBF-BacLBR. Specificity tests of the primer set using 52 phenotypically characterized strains and 75 isolates from rat feces showed only one case each of false-positive and falsenegative. The detection limit of the leuB-directed PCR using BacLBF and BacLBR was 3.9×103colony-forming units. These results indicate that leuB amplification using BacLBF and BacLBR is a useful tool for rapid diagnosis of Bacteriodes infection and for rapid differential diagnosis of anaerobic infections.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
52
1-2
開始ページ
101
終了ページ
108
並び順
101
発行日
2005-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系