ID 110797
著者
マザキ, マサノリ Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
片岡, 佳子 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キノウチ, タケミ Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
ヤマダ, マサミ Division of Genetics and Mutagenesis, Biological Safety Research Center, National Institute of Health Sciences
ノウミ, タケヒコ Division of Genetics and Mutagenesis, Biological Safety Research Center, National Institute of Health Sciences
クワハラ, トモミ Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
アキモト, シゲル Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
大西, 克成 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧
キーワード
caraway
MNNG
antimutagenicity
O6-methylguanine
資料タイプ
学術雑誌論文
抄録
To elucidate the mechanism of antimutagenicity of caraway, we examined the effects of caraway seed extract on N-methyl-N’-nitro-N-nitrosoguanidine (MNNG)-induced mutagenesis in DNA methyltransferase-deficient Salmonella typhimurium strains, O6-methylguanineDNA adduct formation, and thiol content in S. typhimurium cells. MNNG was highly mutagenic for ogt- strains YG7104(ogt- ada+) and YG7108 (ogt-ada-), and it showed slightly higher mutagenicity in strain YG7100 (ogt+ ada-) than in strains TA100 and TA1535. Hot water extract of caraway seeds inhibited MNNG-induced mutation only in the ogt+ strains. In the presence of caraway extract, O6-methylguanine DNA adducts in strain YG7100 were decreased in proportion to the decrease of MNNG-induced mutagenesis. Although MNNG is known to degrade in the presence of thiols to produce methyl cation which can react with DNA, caraway had no effect on cellular concentrations of acid-soluble thiols. These results indicate that caraway does not directly inactivate MNNG and that Ogt-O6-methylguanine-DNAmethyltransferasemay be involved in the antimutagenic activity of caraway.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
53
1-2
開始ページ
123
終了ページ
133
並び順
123
発行日
2006-02
備考
p.123 所属表記に誤記あり(誤)3.・・・Biologica 1 Safety Research Center, ・・・→(正)3.・・・Biological Safety Research Center, ・・・
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系