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ID 111094
タイトル別表記
緑膿菌クオラムセンシングオートインデューサー類似化合物は耐性菌に対し抗菌薬の活性を増強する
Enhancement activity of QS autoinducer analog
著者
天羽, 崇 徳島大学大学院口腔科学教育部(口腔科学専攻) KAKEN研究者をさがす
Kariyama, Reiko Okayama University|Okayama Gakuin University
Hori, Kenji Okayama University
Irie, Yasuhiko University of Dayton
Viducic, Darija Tokushima University
Igarashi, Jun Otsuka Chemical
Suga, Hiroaki the University of Tokyo
Kumon, Hiromi Okayama University
キーワード
Pseudomonas aeruginosa
CRE
antibiotic resistance
資料タイプ
学位論文
抄録
In this study, we have investigated the effects of the newly synthesized analog of Pseudomonas aeruginosa quorum-sensing autoinducer named AIA-1 (autoinducer analog) against antibiotic-resistant bacteria. In vitro susceptibility and killing assays for P. aeruginosa PAO1ΔoprD mutant and clinical isolates were performed by using antibiotics and AIA-1. In an in vivo assay, a luminescent carbapenem-resistant strain derived from PAO1ΔoprD was injected into neutropenic ICR mice and bioluminescence images were acquired after the treatment with antibiotics and AIA-1. Additionally, we investigated the effects of the combination use against carbapenem- resistant Enterobacteriaceae (CRE). Using killing assays in P. aeruginosa, the survival rates in the presence of antibiotics and AIA-1 significantly decreased in comparison with those with antibiotics alone. Furthermore, dual treatment of biapenem and AIA-1 was more effective than biapenem alone in a mouse infection model. AIA-1 did not change the MICs in P. aeruginosa, suggesting that AIA-1 acts on the mechanism of antibiotic tolerance. Conversely, the MICs of antibiotics decreased in the presence of AIA-1 in some CRE strains, indicating that AIA-1 may require additional mechanism to act on CRE. In conclusion, AIA-1 may be a potent drug for clinical treatment of infections caused by antibiotic-resistant bacteria.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
64
1-2
開始ページ
101
終了ページ
109
並び順
101
発行日
2017-02
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704261006.pdf
審査要旨:LID201704261007.pdf
論文本文:LID201704261008.pdf
本論文は,著者Takashi Amohの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3078号
学位記番号
甲口第421号
学位授与年月日
2017-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系