ID 111178
タイトル別表記
マウス肺気腫モデルにおける胎仔肺移植による肺再生
Fetal tissue for mouse lung regeneration
著者
宇山, 攻 徳島大学大学院医科学教育部(医学専攻)
先山, 正二 The University of Tokushima KAKEN研究者をさがす
監﨑, 孝一郎 The University of Tokushima
奥村, 和正 The University of Tokushima KAKEN研究者をさがす
キーワード
Pallid mouse
COPD
lung regeneration
fetal tissue
pulmonary emphysema
資料タイプ
学位論文
抄録
The mortality and morbidity of chronic obstructive pulmonary disease are high. However, no radical therapy has been developed to date. The purpose of this study was to evaluate whether fetal mouse lung tissue can grow and differentiate in the emphysematous lung. Fetal lung tissue from green fluorescent protein C57BL/6 mice at 16 days’ gestation was used as donor material. Twelve-month-old pallid mice were used as recipients. Donor lungs were cut into small pieces and implanted into the recipient left lung by performing thoracotomy under anesthesia. The recipient mice were sacrificed at day 7, 14, and 28 after implantation and used for histological examination. Well-developed spontaneous pulmonary emphysema was seen in 12-month-old pallid mice. Smooth and continuous connection between implanted fetal lung tissue and recipient lung was recognized. Air space expansion and donor tissue differentiation were observed over time. We could clearly distinguish the border zones between injected tissue and native tissue by the green fluorescence of grafts. Fetal mouse lung fragments survived and differentiated in the emphysematous lung of pallid mice. Implantation of fetal lung tissue in pallid mice might lead to further lung regeneration research from the perspective of respiratory and exercise function.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
63
3-4
開始ページ
182
終了ページ
186
並び順
182
発行日
2016-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201610171001.pdf
論文本文 : LID201610171002.pdf
本論文は,著者Koh Uyamaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2963号
学位記番号
甲医第1294号
学位授与年月日
2016-07-28
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院