ID | 111281 |
タイトル別表記 | フクロネズミ腎近位尿細管細胞におけるナトリウム依存性リン酸トランスポーターNaPi-IIcと細胞内vacuole形成の関連
NaPi-IIc induces vacuole formation in OK cells
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著者 |
Ohnishi, Saori
University of Tokushima
Ohi, Akiko
University of Tokushima
Ito, Mikiko
University of Hyogo
Kido, Shinsuke
Kinki University
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キーワード | proximal tubule
SLC34A3
opossum kidney cells
proliferation
vacuole
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資料タイプ |
学位論文
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抄録 | NaPi-IIc/SLC34A3 is a sodium-dependent inorganic phosphate (Pi) transporter in the renal proximal tubules and its mutations cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH). In the present study, we created a specific antibody for opossum SLC34A3, NaPi-IIc (oNaPi-IIc), and analyzed its localization and regulation in opossum kidney cells (a tissue culture model of proximal tubular cells). Immunoreactive oNaPi-IIc protein levels increased during the proliferative phase and decreased during differentiation. Moreover, stimulating cell growth upregulated oNaPi-IIc protein levels, whereas suppressing cell proliferation downregulated oNaPi-IIc protein levels. Immunocytochemistry revealed that endogenous and exogenous oNaPi-IIc proteins localized at the protrusion of the plasma membrane, which is a phosphatidylinositol 4,5-bisphosphate (PIP2) rich-membrane, and at the intracellular vacuolar membrane. Exogenous NaPi-IIc also induced cellular vacuoles and localized in the plasma membrane. The ability to form vacuoles is specific to electroneutral NaPi-IIc, and not electrogenic NaPi-IIa or NaPi-IIb. In addition, mutations of NaPi-IIc (S138F and R468W) in HHRH did not cause cellular PIP2-rich vacuoles. In conclusion, our data anticipate that NaPi-IIc may regulate PIP2 production at the plasma membrane and cellular vesicle formation.
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掲載誌名 |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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cat書誌ID | AA11166929
AA12022913
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出版者 | Faculty of Medicine Tokushima University
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巻 | 62
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号 | 3-4
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開始ページ | 209
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終了ページ | 218
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並び順 | 209
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発行日 | 2015-08
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備考 | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201508051001.pdf 論文本文 : LID201509301001.pdf 本論文は,著者Yuji Shiozakiの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第2867号
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学位記番号 | 甲栄第230号
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学位授与年月日 | 2015-07-23
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学位名 |
博士(栄養学)
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学位授与機関 |
徳島大学
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部局 |
医学系
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