ID | 111308 |
著者 |
Karabasil, Mileva Ratko
The University of Tokushima
Azlina, Ahmad
The University of Tokushima
Purwanti, Nunuk
The University of Tokushima
Purevjav, Javkhlan
The University of Tokushima
姚, 陳娟
The University of Tokushima
|
キーワード | aquaporin-5
PKA-consensus sequence
trafficking
|
資料タイプ |
学術雑誌論文
|
抄録 | Three constructs having mutated PKA-target motif at 152SRRTS of AQP5, an exocrine type water channel, were prepared and fused to C-terminus of green fluorescence protein cDNA to examine the effects of blocking of phosphorylation at 152SRRTS (a consensus PKA-target motif of AQP5) on translocation or trafficking of the chimeric proteins expressed in the Madin-Darby canine kidney-II (MDCK-II) cells. H-89 treatment increased translocation of wild-type GFP-AQP5 to the apical membrane. All 3 mutant molecules translocated 1.5 to 2 times more than the control wild-type GFP-AQP5. Colchicine but not cytochalasin B inhibited the translocation of wild-type GFP-AQP5. Present results suggest dephosphorylation of this consensus sequence increase GFP-AQP5 translocation, and that microtubules but not microfilaments are involved in this event.
|
掲載誌名 |
The Journal of Medical Investigation
|
ISSN | 13496867
13431420
|
cat書誌ID | AA11166929
AA12022913
|
出版者 | Faculty of Medicine Tokushima University
|
巻 | 56
|
号 | 1-2
|
開始ページ | 55
|
終了ページ | 63
|
並び順 | 55
|
発行日 | 2009-02
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
出版社版
|
部局 |
歯学系
生物資源系
|