ID 111353
著者
Guo, Ming-Yu Nihon University
Narita, Takanori Nihon University
Qi, Bing Nihon University
Satoh, Keitaro Nihon University
Katsumata-Kato, Osamu Nihon University
Matsuki-Fukushima, Miwako Nihon University
Fujita-Yoshigaki, Junko Nihon University
Sugiya, Hiroshi Nihon University
キーワード
oxidative stress
β-receptor
diamide
amylase release
parotid acinar cells
資料タイプ
学術雑誌論文
抄録
In parotid acinar cells, activation of β-adrenergic receptors provokes exocytotic amylase release via the accumulation of intracellular cAMP. Cellular redox status plays a pivotal role in the regulation of various cellular functions. Cellular redox imbalance caused by the oxidation of cellular antioxidants, as a result of oxidative stress, induces significant biological damages. In this study, we examined effect of diamide, a thioloxidizing reagent, on amylase release in rat parotid acinar cells. In the presence of diamide, isoproterenol (IPR)-induced cAMP formation and amylase release were partially reduced. Diamide had no effect on amylase release induced by forskolin and mastoparan, an adenylate cyclase activator and heterotrimeric GTP binding protein activator, respectively. In the cells pretreated with diamide, the binding affinity of [3H]dihydroalprenolol to β-receptors was reduced. These results suggest that oxidative stress results in reduction of binding affinity of ligand on β-receptor and consequently reduces protein secretory function in rat parotid acinar cells.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
Supplement
開始ページ
284
終了ページ
286
並び順
284
発行日
2009-12
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版