ID 111487
著者
Hashimoto, Sadamitsu Tokyo Dental College
Murakami, Masataka National Institutes of Natural Sciences
キーワード
submandibular gland
paracellular pathway
fluid secretion
tight junction
cytoskeleton
freeze-fracture
資料タイプ
学術雑誌論文
抄録
The morphological change of the paracellular route for fluid secretion is still a long-standing question. The purpose of this study was to visualize alterations in the cytoskeleton structure of tight junctions caused by carbachol (CCh) and isoproterenol (IPR) treatment of perfused rat submandibular glands (SMGs), using freeze-fracture (FF) replicas of rapidly frozen tissues. Isolated SMGs from male Wistar rats were perfused and stimulated with 1 μM CCh and IPR. Specimens were immediately rapidly frozen with liquid helium by metal contact. After cutting and deep etching, FF replicas were obtained by rotary shadowing and were examined by transmission electron microscopy. After CCh/ IPR stimulation, the strand particles of TJs rearranged with free ends and terminal loops. In the vertical fracture surface, cytoskeletal filaments beneath the plasma membrane were arranged in a thicker layer than those of the gland without stimulation. Contraction of the submembranous actin cytoskeleton during exocytosis elicited by CCh/IPR may cause rearrangement of TJ strands due to direct interactions between the TJ membrane particles and actin filaments via the tiny bridging structures. The rearrangement and movement of TJ membrane particles involves reconstruction of the subluminal membranous actin filament network through the intermediary of interstitial molecules and may modulate increased paracellular permeability after CCh/IPR stimulation.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
Supplement
開始ページ
395
終了ページ
397
並び順
395
発行日
2009-12
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版