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ID 111862
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Isolation of Acanthamoeba with Sabouroud's glucose agar plate
著者
加藤, 俊彦 屋島総合病院
三村, 康男 徳島大学
キーワード
Acanthamoeba
non-nutrient agar plate
Sabouroud's glucose agar plate
trophozoite
cyst
資料タイプ
学術雑誌論文
抄録
Isolation of Acanthamoeba or finding out the cysts from corneal leasions is necessary to confirm the diagnosis of Acanthamoeba keratitis. However, we cannot always find out the cyst from corneal scrapings of the lesions. In these cases the isolation of Acanthamoeba is very important to confirm the diagnosis.
Non-nutrient agar plates are generally used to isolate Acanthamoeba, but it is difficult to get them easily at many hospitals.
To find out another agar that can be used to isolate Acanthamoeba and got easily, Sabouroud's glucose agar plates were compared with non-nutrient agar plates for the isolation of Acanthamoeba.
Both agar plates seeded with Escherichia coli showed a nice growth of trophozoites first and cysts later. A clinical material, stock solution of contact lens used by a suspected patient of Acanthamoeba keratitis, was put on Sabouroud's glucose agar plate seeded with Escherichia coli. After three days of incubation at 25°C Acanthamoeba was found on the plate.
Sabouroud's glucose agar plate was a very useful agar plate for the isolation of Acanthamoeba.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
52
2
開始ページ
40
終了ページ
45
並び順
40
発行日
1996-04-25
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
医学系