ID 111979
タイトル別表記
Zinc-dependent and independent actions of hydroxyhydroquinone
著者
Wada, Honoka Tokushima University
Oyama, Keisuke Osaka University
Kamae, Risa Tokushima University
キーワード
hydroxyhydroquinone
zinc
oxidative stress
glutathione
lymphocytes
資料タイプ
学術雑誌論文
抄録
Coffee contains hydroxyhydroquinone (HHQ). HHQ is one of by-products released during bean roasting. Therefore, it is important to elucidate the bioactivity of HHQ to predict its beneficial or adverse effects on humans. We studied zinc-dependent and independent actions of commercially-procured synthetic HHQ in rat thymocytes using flow cytometric techniques with propidium iodide, FluoZin-3-AM, 5-chloromethylfluorescein diacetate, and annexin V-FITC. HHQ at 1050 μM elevated intracellular Zn2+ levels by releasing intracellular Zn2+. HHQ at 10 μM increased cellular thiol content in a Zinc-dependent manner. However, HHQ at 30–50 μM reduced cellular thiol content. Although the latter actions of HHQ (30–50 μM) were suggested to increase cell vulnerability to oxidative stress, HHQ at 0.3–100 μM significantly protected cells against oxidative stress induced by H2O2. The process of cell death induced by H2O2 was delayed by HHQ, although both H2O2 and HHQ increased the population of annexin V-positive living cells. However, HHQ at 10–30 μM promoted cell death induced by A23187, a calcium ionophore. HHQ at 10–30 μM exerted contrasting effects on cell death caused by oxidative stress and Ca2+ overload. Because HHQ is considered to possess diverse cellular actions, coffee with reduced amount of HHQ may be preferable to avoid potential adverse effects.
掲載誌名
Drug and Chemical Toxicology
ISSN
01480545
15256014
cat書誌ID
AA00161752
出版者
Taylor & Francis
42
5
開始ページ
496
終了ページ
501
発行日
2018-02-09
備考
This is an Accepted Manuscript of an article published by Taylor & Francis in Drug and Chemical Toxicology on 09 Feb 2018, available online: http://www.tandfonline.com/10.1080/01480545.2018.1429462.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系