ID 111980
タイトル別表記
DCOIT and Neurotransmission
著者
Wakita, Masahito Kumamoto Kinoh Hospital|Kumamoto Health Science University
Shoudai, Kiyomitsu Kumamoto Health Science University
Akaike, Norio Kumamoto Kinoh Hospital|Kumamoto University
キーワード
4,5-Dichloro-2-octyl-4-isothiazolin-3-one (DCOIT)
Neurotransmission
Synaptic currents
GABA
Glutamate
Ca2+
資料タイプ
学術雑誌論文
抄録
4,5-Dichloro-2-octyl-4-isothiazolin-3-one (DCOIT) is an alternative to organotin antifoulants, such as tributyltin and triphenyltin. Since DCOIT is found in harbors, bays, and coastal areas worldwide, this chemical compound may have some impacts on ecosystems. To determine whether DCOIT possesses neurotoxic activity by modifying synaptic transmission, we examined the effects of DCOIT on synaptic transmission in a ‘synaptic bouton’ preparation of rat brain. DCOIT at concentrations of 0.03–1 μM increased the amplitudes of evoked synaptic currents mediated by GABA and glutamate, while it reduced the amplitudes of these currents at 3–10 μM. However, the currents elicited by exogenous applications of GABA and glutamate were not affected by DCOIT. DCOIT at 1–10 μM increased the frequency of spontaneous synaptic currents mediated by GABA. It also increased the frequency of glutamate-mediated spontaneous currents at 0.3–10 μM. The frequencies of miniature synaptic currents mediated by GABA and glutamate, observed in the presence of tetrodotoxin under external Ca2+-free conditions, were increased by 10 μM DCOIT. With the repetitive applications of DCOIT, the frequency of miniature synaptic currents mediated by glutamate was not increased by the second and third applications of DCOIT. Voltage-dependent Ca2+ channels were not affected by DCOIT, but DCOIT slowed the inactivation of voltage-dependent Na+ channels. These results suggest that DCOIT increases Ca2+ release from intracellular Ca2+ stores, resulting in the facilitation of both action potential-dependent and spontaneous neurotransmission, possibly leading to neurotoxicity.
掲載誌名
Chemosphere
ISSN
00456535
cat書誌ID
AA00603442
AA11523866
出版者
Elsevier
184
開始ページ
337
終了ページ
346
発行日
2017-05-29
備考
論文本文は2019-05-29以降公開予定
権利情報
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
324725
出版社版DOI
出版社版URL
言語
eng
著者版フラグ
その他
部局
生物資源系