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ID 111985
著者
Nishimura-Danjobara, Yumiko Tokushima University|Hiroshima University
Oyama, Keisuke Tokushima University|Osaka University
キーワード
N-(3-oxododecanoyl)-L-homoserine-Lactone
Lymphocytes
Membrane potential
Membrane K+ permeability
K+ channels
資料タイプ
学術雑誌論文
抄録
To study the adverse effects of N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule, on mammalian host cells, its effect on membrane potential was examined in rat thymic lymphocytes using flow cytometric techniques with a voltage-sensitive fluorescent probe. As 3–300 μM ODHL elicited hyperpolarization, it is likely that it increases membrane K+ permeability because hyperpolarization is directly linked to changing K+ gradient across membranes, but not Na+ and Cl- gradients. ODHL did not increase intracellular Ca2+ concentration. ODHL also produced a response in the presence of an intracellular Zn2+ chelator. Thus, it is unlikely that intracellular Ca2+ and Zn2+ are attributed to the response. Quinine, a non-specific K+ channel blocker, greatly reduced hyperpolarization. However, because charybdotoxin, tetraethylammonium chloride, 4-aminopyridine, and glibenclamide did not affect it, it is pharmacologically hypothesized that Ca2+-activated K+ channels, voltage-gated K+ channels, and ATP-sensitive K+ channels are not involved in ODHL-induced hyperpolarization. Although the K+ channels responsible for ODHL-induced hyperpolarization have not been identified, it is suggested that ODHL can elicit hyperpolarization in mammalian host cells, disturbing cellular functions.
掲載誌名
Chemico-Biological Interactions
ISSN
00092797
cat書誌ID
AA0060252X
AA11523855
出版者
Elsevier
283
開始ページ
91
終了ページ
96
発行日
2018-02-08
権利情報
© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系