ID | 111985 |
著者 |
Nishimura-Danjobara, Yumiko
Tokushima University|Hiroshima University
Oyama, Keisuke
Tokushima University|Osaka University
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キーワード | N-(3-oxododecanoyl)-L-homoserine-Lactone
Lymphocytes
Membrane potential
Membrane K+ permeability
K+ channels
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資料タイプ |
学術雑誌論文
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抄録 | To study the adverse effects of N-(3-oxododecanoyl)-L-homoserine-lactone (ODHL), a quorum sensing molecule, on mammalian host cells, its effect on membrane potential was examined in rat thymic lymphocytes using flow cytometric techniques with a voltage-sensitive fluorescent probe. As 3–300 μM ODHL elicited hyperpolarization, it is likely that it increases membrane K+ permeability because hyperpolarization is directly linked to changing K+ gradient across membranes, but not Na+ and Cl- gradients. ODHL did not increase intracellular Ca2+ concentration. ODHL also produced a response in the presence of an intracellular Zn2+ chelator. Thus, it is unlikely that intracellular Ca2+ and Zn2+ are attributed to the response. Quinine, a non-specific K+ channel blocker, greatly reduced hyperpolarization. However, because charybdotoxin, tetraethylammonium chloride, 4-aminopyridine, and glibenclamide did not affect it, it is pharmacologically hypothesized that Ca2+-activated K+ channels, voltage-gated K+ channels, and ATP-sensitive K+ channels are not involved in ODHL-induced hyperpolarization. Although the K+ channels responsible for ODHL-induced hyperpolarization have not been identified, it is suggested that ODHL can elicit hyperpolarization in mammalian host cells, disturbing cellular functions.
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掲載誌名 |
Chemico-Biological Interactions
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ISSN | 00092797
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cat書誌ID | AA0060252X
AA11523855
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出版者 | Elsevier
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巻 | 283
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開始ページ | 91
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終了ページ | 96
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発行日 | 2018-02-08
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権利情報 | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
出版社版DOI | |
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フルテキストファイル | |
言語 |
eng
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著者版フラグ |
著者版
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部局 |
生物資源系
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