ID 112045
タイトル別表記
Cell Biological Approaches
著者
キーワード
trichloroacetic acid(TCA)fixation
Rho
cytokinesis
epithelial sheets
資料タイプ
学術雑誌論文
抄録
Our cell biological approaches are introduced in this article. First, we show development of a new fixation protocol using trichloroacetic acid (TCA) that is ideal for immunofluorescence microscopy detecting cellular distribution of phosphorylated ERM (ezrin/radixin/moesin) proteins. TCA denatures proteins differently from organic solvents or aldehydes and can be a good alternative to conventional fixatives when immunostaining is not successful. Second, using this new fixation protocol, the small GTPase, Rho was found to be successfully immunostained and this led to understanding of molecular mechanism for determination of the position of the cleavage furrow at cytokinesis through microtubules of the mitotic apparatus. Rho activator is translocated to the presumptive furrow region through microtubules, then, Rho is accumulated and activated there leading to myosin II contractility for furrowing. Third, we show the cell ablation using laser beam during microscopic observation. This enables us to analyze cell responses to the death of neighboring cells in epithelial sheets at early stages.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
73
5-6
開始ページ
241
終了ページ
248
並び順
241
発行日
2017-12-25
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
医学系