ID 112430
著者
Ozone, Chikafumi RIKEN|Nagoya University
Suga, Hidetaka Nagoya University
Eiraku, Mototsugu RIKEN
Kadoshima, Taisuke RIKEN
Takata, Nozomu RIKEN
Oiso, Yutaka Nagoya University
Tsuji, Takashi RIKEN
Sasai, Yoshiki RIKEN
資料タイプ
学術雑誌論文
抄録
Anterior pituitary is critical for endocrine systems. Its hormonal responses to positive and negative regulators are indispensable for homeostasis. For this reason, generating human anterior pituitary tissue that retains regulatory hormonal control in vitro is an important step for the development of cell transplantation therapy for pituitary diseases. Here we achieve this by recapitulating mouse pituitary development using human embryonic stem cells. We find that anterior pituitary self-forms in vitro following the co-induction of hypothalamic and oral ectoderm. The juxtaposition of these tissues facilitated the formation of pituitary placode, which subsequently differentiated into pituitary hormone-producing cells. They responded normally to both releasing and feedback signals. In addition, after transplantation into hypopituitary mice, the in vitro-generated corticotrophs rescued physical activity levels and survival of the hosts. Thus, we report a useful methodology for the production of regulator-responsive human pituitary tissue that may benefit future studies in regenerative medicine.
掲載誌名
Nature Communications
ISSN
20411723
cat書誌ID
AA12645905
出版者
Springer Nature
7
開始ページ
10351
発行日
2016-01-14
備考
Supplementary Information : ncomms_7_10351_s1.pdf
Supplementary movies : ncomms_7_10351_s2.mov, ncomms_7_10351_s3.mov
権利情報
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
EDB ID
315722
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系