ID | 112465 |
著者 |
Sato, Keita
Okayama University
Yamashita, Takahiro
Kyoto University
Ohuchi, Hideyo
Okayama University
Takeuchi, Atsuko
Kobe Pharmaceutical University
Gotoh, Hitoshi
Kyoto Prefectural University of Medicine
Ono, Katsuhiko
Kyoto Prefectural University of Medicine
Mizuno, Misao
Osaka University
Mizutani, Yasuhisa
Osaka University
友成, さゆり
Tokushima University
Sakai, Kazumi
Kyoto University
Imamoto, Yasushi
Kyoto University
Wada, Akimori
Kobe Pharmaceutical University
Shichida, Yoshinori
Kyoto University|Ritsumeikan University
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資料タイプ |
学術雑誌論文
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抄録 | Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and as a chromophore. Opsins’ main established mechanism is light-triggered activation through retinal 11-cis-to-all-trans photoisomerization. Here we report a vertebrate non-visual opsin that functions as a Gi-coupled retinal receptor that is deactivated by light and can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all-trans-retinal. More interestingly, the light-induced deactivation through retinal trans-to-cis isomerization is followed by formation of a covalent adduct between retinal and a nearby cysteine, which breaks the retinal-conjugated double bond system, probably at the C11 position, resulting in thermal re-isomerization to all-trans-retinal. Thus, Opn5L1 acts as a reverse photoreceptor. We conclude that, like vertebrate rhodopsin, Opn5L1 is a unidirectional optical switch optimized from an ancestral bidirectional optical switch, such as invertebrate rhodopsin, to increase the S/N ratio of the signal transduction, although the direction of optimization is opposite to that of vertebrate rhodopsin.
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掲載誌名 |
Nature Communications
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ISSN | 20411723
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cat書誌ID | AA12645905
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出版者 | Springer Nature
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巻 | 9
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開始ページ | 1255
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発行日 | 2018-03-28
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備考 | Supplementary Information : ncomms_9_1255_s1.pdf
Peer Review File : ncomms_9_1255_s2.pdf |
権利情報 | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
技術支援部
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