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ID 112841
タイトル別表記
リアルタイムイメージングによる栄養チューブ内における流動食残渣と微生物増殖の検出
著者
山岡, 一平 Otsuka Pharmaceutical Factory, Inc.
Kagawa, Tomohiro Otsuka Pharmaceutical Factory, Inc.
Mizugai, Kazuya Otsuka Pharmaceutical Factory, Inc.
Ebisu, Goro Otsuka Pharmaceutical Factory, Inc.
キーワード
bacteria
biofilms
bioluminescence
enteral nutrition
feeding tubes
fluorescence
資料タイプ
学位論文
抄録
Background:
Enteral nutrition (EN) residues that persist in feeding tubes provide substrates for microorganisms to proliferate and occlude the tubes. Visible EN residues in tubes are easily identified, but smaller residues can persist. We developed a new imaging technique to visualize EN residues and proliferation of microorganisms in feeding tubes.
Materials and Methods:
Feeding tubes containing EN labelled with fluorescent dye and EN with or without various types or amounts of thickeners were flushed once with water and then the tubes were seeded with Pseudomonas aeruginosa Xen5 with recombinant luciferase DNA. Because EN fluoresces intrinsically, EN in the feeding tubes without fluorescent dye was repeatedly flushed until the intrinsic fluorescence levels reached background levels. Fluorescent images of EN residues and bioluminescent images of microorganisms were acquired using an optical imaging system.
Results:
Fluorescence images showed that the amount of EN residues increased at various sites in tubes depending on EN viscosity and the thickening agent, and bioluminescence images showed that microorganism proliferation was associated with a commensurate increase in EN residues. The intrinsic fluorescence of EN also enabled the detection of EN residues in tubes even in the absence of fluorescence dye. Higher EN viscosity required more flushes to reach undetectable levels.
Conclusion:
EN residues and microorganism proliferation in enteral feeding tubes were detected on fluorescence and bioluminescence images, respectively. This simplified approach allowed the real-time visualization of EN residues and microorganisms in feeding tubes.
掲載誌名
Nutrtion in Clinical Practice
ISSN
19412452
出版者
Wiley|American Society for Parenteral and Enteral Nutrition
32
2
開始ページ
282
終了ページ
287
発行日
2016-11-04
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Ippei Yamaokaの学位論文として提出され, 学位審査・授与の対象となっている。
This is the peer reviewed version of the following article: Yamaoka, I. , Kagawa, T. , Mizugai, K. and Ebisu, G. (2017), Detecting Enteral Nutrition Residues and Microorganism Proliferation in Feeding Tubes via Real‐Time Imaging. Nutrition in Clinical Practice, 32: 282-287, which has been published in final form at https://doi.org/10.1177/0884533616675189. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
乙第2104号
学位記番号
乙栄第95号
学位授与年月日
2018-09-27
学位名
博士(栄養学)
学位授与機関
徳島大学