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ID 113212
タイトル別表記
サイトカイン誘発好中球化学誘引物質の発現はTNFα発現を抑制することにより卵胞の閉鎖およびアポトーシスから回避させる
著者
田中, 優 Japanese Red Cross Kochi Hospital KAKEN研究者をさがす
Ushigoe, Kenjiro The University of Tokushima
Yano, Yuya The University of Tokushima
Taniguchi, Yuka The University of Tokushima
松崎, 利也 The University of Tokushima KAKEN研究者をさがす
キーワード
Apoptosis
follicle
ovary
ovulation
tumor necrosis factor alpha
資料タイプ
学位論文
抄録
Aim: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined.
Methods: Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax).
Results: The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration.
Conclusion: These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
掲載誌名
Reproductive Medicine and Biology
ISSN
14470578
cat書誌ID
AA11706516
出版者
Wiley|Japan Society for Reproductive Medicine
16
2
開始ページ
157
終了ページ
165
発行日
2017-03-12
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Yu Tanakaの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
© 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
乙第2107号
学位記番号
乙医第1756号
学位授与年月日
2019-03-19
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院