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ID 113298
著者
Hirata, Yohko Tokushima University
保坂, 利男 Tokushima University
Le, Chung T.K. Tokushima University
Jambaldorj, Bayasgalan Tokushima University
Teshigawara, Kiyoshi Tokushima University
キーワード
Vimentin
GLUT4
IRAP
資料タイプ
学術雑誌論文
抄録
Insulin-responsive aminopeptidase (IRAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs.
掲載誌名
Biochemical and Biophysical Research Communications
ISSN
0006291X
cat書誌ID
AA00564395
出版者
Elsevier
405
1
開始ページ
96
終了ページ
101
発行日
2011-01-07
備考
本論文は,著者Yohko Hirataの学位論文として提出され,学位審査・授与の対象となっている。
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出版社版DOI
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フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系
医学系