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ID 113344
著者
Kikuchi, Yusaku The University of Tokushima
古川, 和寛 The University of Tokushima KAKEN研究者をさがす
キーワード
Oligonucleotide
2'-Modified-4'-thioRNA
Gene suppression
U1 snRNA interference
資料タイプ
学術雑誌論文
抄録
Gene suppression via U1 small nuclear RNA interference (U1i) is considered to be one of the most attractive approaches, and takes the place of general antisense, RNA interference (RNAi), and anti-micro RNA machineries. Since the U1i can be induced by short oligonucleotides (ONs), namely U1 adaptors consisting of a ‘target domain’ and a ‘U1 domain’, we prepared adaptor ONs using 2'-modified-4'-thionucleosides developed by our group, and evaluated their U1i activity. As a result, the desired gene suppression via U1i was observed in ONs prepared as a combination of 2'-fluoro-4'-thionucleoside and 2'-fluoronucleoside units as well as only 2'-fluoronucleoside units, while those prepared as combination of 2'-OMe nucleoside/2'-OMe-4'-thionucleoside and 2'-fluoronucleoside units did not show significant activity. Measurement of Tm values indicated that a higher hybridization ability of adaptor ONs with complementary RNA is one of the important factors to show potent U1i activity.
掲載誌名
Bioorganic & Medicinal Chemistry
ISSN
09680896
cat書誌ID
AA10938083
AA11522681
出版者
Elsevier
21
17
開始ページ
5292
終了ページ
5296
発行日
2013-06-18
権利情報
©2013 The Authors. Published by Elsevier Ltd.Open access under CC BY-NC-ND license.(https://creativecommons.org/licenses/by-nc-nd/3.0/)
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
薬学系