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ID 113362
タイトル別表記
Effect of Short-term TNF-α-stimulation of MC3T3-E1
著者
Naritani, Mio Tokushima University
Raju, Resmi Tokushima University
Oshima, Masamitsu Tokushima University
Inoue, Masahisa Tokushima Bunri University
キーワード
TNF-α
Osteoblast-like cell (MC3T3-E1)
Cell proliferation
Cell differentiation
資料タイプ
学術雑誌論文
抄録
Tumor necrosis factor-alpha (TNF-α) is an inflammatory cytokine known to cause bone resorption, swelling and edema during tissue organization. Conversely, TNF-α has also been shown to participate in tissue regeneration during the wound healing process. We have previously investigated the effects of TNF-α on human dental pulp cell differentiation. Dental pulp cells are composed of different cell types including primary odontoblasts and fibroblasts. We determined that the ratio of stem cells within the pulp cell population was increased following short-term stimulation with TNF-α. The aim of this study therefore was to investigate the effect of short-term stimulation with TNF-α on osteoblast-like MC3T3-E1 cell growth and differentiation. MC3T3-E1 cells were cultured in standard growth medium and on reaching sub-confluence were exposed to recombinant TNF-α (10 and 100 ng/ml) for 2 days prior to assessing their cell proliferation and differentiation properties in comparison to non-stimulated MC3T3-E1 cells (control). Although no significant differences in cell proliferation were observed between the TNF-α-stimulated and control groups, cell differentiation was delayed in the TNF-α-stimulated groups. In summary, short-term stimulation of cultured MC3T3-E1 cells with TNF-α had only minimal effect on their growth and differentiation.
掲載誌名
Journal of Hard Tissue Biology
ISSN
13417649
1880828X
cat書誌ID
AA11074332
出版者
The Society for Hard Tissue Regenerative Biology
27
3
開始ページ
213
終了ページ
218
発行日
2018-07-01
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系
病院