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ID 113372
タイトル別表記
TNF-α短期刺激による骨髄由来間葉系幹細胞の細胞動態解析
Effect of Short Term TNF-α Stimulation of BMSCs
著者
成谷, 美緒 徳島大学大学院口腔科学教育部(口腔科学専攻)
Raju, Resmi Tokushima University
キーワード
TNF-α
Bone marrow-derived mesenchymal stem cells
Short-term stimulation
Cell proliferation
Cell differentiation
資料タイプ
学位論文
抄録
Bone marrow-derived mesenchymal stem cells (BMSCs) have considerable potential for self-renewal and multi-differentiation. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine and is involved in tissue regeneration during wound healing. It was already reported that cultured human dental pulp cells acquire stem cell properties following short-term stimulation by TNF-α. However, it has not been clarified if BMSCs acquire stem cell properties after TNF-α treatment. The purpose of this study was to investigate the effect of short-term stimulation with TNF-α on BMSCs. Rat BMSCs were cultured up to 60% confluence and then incubated with 1–100 ng/ml of recombinant rat TNF-α (rTNF-α) for a further 2 days. After reaching subconfluence, cells were passaged once to remove rTNF-α completely before subsequent assays. Cells in the control group were passaged without stimulation. Expression levels of Nanog and Oct4 stem cell markers were significantly increased in the rTNF-α 10 ng/ml stimulation group. rTNF-α stimulation did not affect cell proliferation compared with the control group. However, rTNF-α stimulation led to a delay in cell differentiation. This study suggests that short-term TNF-α stimulation of BMSCs significantly increased their stem cell phenotype, but delayed their osteogenic cell differentiation.
掲載誌名
Journal of Hard Tissue Biology
ISSN
13417649
1880828X
cat書誌ID
AA11074332
出版者
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
28
2
開始ページ
99
終了ページ
108
発行日
2019-04-24
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mio Naritaniの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3282号
学位記番号
甲口第447号
学位授与年月日
2019-03-22
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
病院