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ID 113682
著者
Morito, Katsuya Tokushima University
Shimizu, Ryota Tokushima University
Kitamura, Nahoko Kyoto University
Park, Si-Bum Kyoto University
Kishino, Shigenobu Kyoto University
Ogawa, Jun Kyoto University
キーワード
Peroxisomal β-oxidation
Hydroxy fatty acid
Linoleic acid
Fatty acid metabolism
Gut lactic acid bacteria
資料タイプ
学術雑誌論文
抄録
Microorganisms in animal gut produce unusual fatty acids from the ingested diet. Two types of hydroxy fatty acids (HFAs), 10-hydroxy-cis-12-octadecenoic acid (HYA) and 10-hydroxy-octadecanoic acid (HYB), are linoleic acid (LA) metabolites produced by Lactobacillus plantarum. In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 hours. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2- and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HYA. The PEX-dependent disappearance was observed in the experiment with HYB, but not with LA. We also found that HYA treatment up-regulates peroxisomal β-oxidation activity of human gastric MKN74 cells and intestinal Caco-2 cells. These results indicate a possibility that HFAs produced from gut bacteria affect lipid metabolism of host via modulation of peroxisomal β-oxidation activity.
掲載誌名
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN
13881981
cat書誌ID
AA11522965
AA11300409
出版者
Elsevier
1864
11
開始ページ
1619
終了ページ
1628
発行日
2019-07-24
権利情報
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
薬学系