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ID 113697
タイトル別表記
Regulation of cricket metamorphosis
Respective roles of Kr-h1, Br and E93 in hemimetabolous metamorphosis
著者
友成, さゆり Tokushima University
キーワード
Gryllus bimaculatus
insect metamorphosis
Krüppel homolog 1
Broad complex
E93
資料タイプ
学術雑誌論文
抄録
Juvenile hormones (JHs) and the genetic interaction between the transcription factors Krüppel homolog 1 (Kr-h1) and Broad (Br) regulate the transformation of insects from immature to adult forms in both types of metamorphosis (holometaboly with a pupal stage versus hemimetaboly with no pupal stage); however, knowledge about the exact instar in which this occurs is limited. Using the hemimetabolous cricket Gryllus bimaculatus (Gb), we demonstrate that a genetic interaction occurs among Gb’Kr-h1, Gb’Br and the adult-specifier transcription factor Gb’E93 from the sixth to final (eighth) nymphal instar. Gb’Kr-h1 and Gb’Br mRNAs were strongly expressed in the abdominal tissues of sixth instar nymphs, with precocious adult moults being induced by Gb’Kr-h1 or Gb’Br knockdown in the sixth instar. Depletion of Gb’Kr-h1 or Gb’Br up-regulates Gb’E93 in the sixth instar. In contrast, Gb’E93 knockdown at the sixth instar prevents nymphs transitioning to adults, instead producing supernumerary nymphs. Gb’E93 also represses Gb’Kr-h1 and Gb’Br expression in the penultimate nymphal instar, demonstrating its important role in adult differentiation. Our results suggest that the regulatory mechanisms underlying the pupal transition in holometabolous insects are evolutionarily conserved in hemimetabolous G. bimaculatus, with the penultimate and final nymphal periods being equivalent to the pupal stage.
掲載誌名
Philosophical Transactions of the Royal Society B : Biological Sciences
ISSN
09628436
14712970
cat書誌ID
AA00772905
出版者
The Royal Society
374
1783
開始ページ
20190225
発行日
2019-08-26
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系
技術支援部