ID | 114016 |
タイトル別表記 | Comparison of 2 expression systems using COS7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1 (CPT1b)
|
著者 |
Hada, Takuya
University of Tokushima
Kato, Yumiko
University of Tokushima
尾華, 絵里子
University of Tokushima
Yamamoto, Atsushi
University of Tokushima
橋本, 満
Matsuyama University
|
キーワード | Carnitine palmitoyltransferase (CPT1)
Expression system
Mitochondria
|
資料タイプ |
学術雑誌論文
|
抄録 | Carnitine palmitoyltransferase 1 (CPT1), catalyzing the transfer of the acyl group from acyl-CoA to carnitine to form acylcarnitine, is located at the outer mitochondrial membrane. Because it is easily inactivated by solubilization, expression systems using living cells are essential for its functional characterization. COS7 cells or yeast cells are often utilized for this purpose; however, the advantages/disadvantages of the use of these cells or the question as to how the CPT1 enzyme expressed by these cells differs are still uncertain. In this study, we characterized the heart/muscle-type isozyme of rat CPT1 (CPT1b) expressed by these 2 cellular expression systems. The mitochondrial fraction prepared from yeast cells expressing CPT1b showed 25% higher CPT1 activity than that obtained from COS7 cells. However, the expression level of CPT1b in the former was 3.8 times lower than that in the latter; and thus, under the present experimental conditions, the specific activity of CPT1b expressed in yeast cells was estimated to be approximately 5 times higher than that expressed in COS7 cells. Possible reasons for this difference are discussed.
|
掲載誌名 |
Protein Expression and Purification
|
ISSN | 10465928
|
cat書誌ID | AA10814149
AA11542088
|
出版者 | Elsevier
|
巻 | 82
|
号 | 1
|
開始ページ | 192
|
終了ページ | 196
|
発行日 | 2012-01-13
|
権利情報 | © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
|
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
著者版
|
部局 |
薬学系
先端酵素学研究所
|