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ID 114016
タイトル別表記
Comparison of 2 expression systems using COS7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1 (CPT1b)
著者
Hada, Takuya University of Tokushima
Kato, Yumiko University of Tokushima
尾華, 絵里子 University of Tokushima
Yamamoto, Atsushi University of Tokushima
橋本, 満 Matsuyama University
キーワード
Carnitine palmitoyltransferase (CPT1)
Expression system
Mitochondria
資料タイプ
学術雑誌論文
抄録
Carnitine palmitoyltransferase 1 (CPT1), catalyzing the transfer of the acyl group from acyl-CoA to carnitine to form acylcarnitine, is located at the outer mitochondrial membrane. Because it is easily inactivated by solubilization, expression systems using living cells are essential for its functional characterization. COS7 cells or yeast cells are often utilized for this purpose; however, the advantages/disadvantages of the use of these cells or the question as to how the CPT1 enzyme expressed by these cells differs are still uncertain. In this study, we characterized the heart/muscle-type isozyme of rat CPT1 (CPT1b) expressed by these 2 cellular expression systems. The mitochondrial fraction prepared from yeast cells expressing CPT1b showed 25% higher CPT1 activity than that obtained from COS7 cells. However, the expression level of CPT1b in the former was 3.8 times lower than that in the latter; and thus, under the present experimental conditions, the specific activity of CPT1b expressed in yeast cells was estimated to be approximately 5 times higher than that expressed in COS7 cells. Possible reasons for this difference are discussed.
掲載誌名
Protein Expression and Purification
ISSN
10465928
cat書誌ID
AA10814149
AA11542088
出版者
Elsevier
82
1
開始ページ
192
終了ページ
196
発行日
2012-01-13
権利情報
© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
薬学系
先端酵素学研究所