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ID 114080
著者
Sternberg, Jenna R. Sorbonne Universités|Harvard University
Prendergast, Andrew E. Sorbonne Universités
Brosse, Lucie Aix Marseille Université
Cantaut-Belarif, Yasmine Sorbonne Universités
Thouvenin, Olivier Sorbonne Universités|PSL Research University
Orts-Del'Immagine, Adeline Sorbonne Universités
Castillo, Laura Aix Marseille Université
Djenoune, Lydia Sorbonne Universités|Massachusetts General Hospital
栗栖, 修作 Riken Brain Science Institute|Tokushima University 徳島大学 教育研究者総覧
McDearmid, Jonathan R. University of Leicester
Bardet, Pierre-Luc Sorbonne Universités
Boccara, Claude PSL Research University
Okamoto, Hitoshi Riken Brain Science Institute
Delmas, Patrick Aix Marseille Université
Wyart, Claire Sorbonne Universités
資料タイプ
学術雑誌論文
抄録
Defects in cerebrospinal fluid (CSF) flow may contribute to idiopathic scoliosis. However, the mechanisms underlying detection of CSF flow in the central canal of the spinal cord are unknown. Here we demonstrate that CSF flows bidirectionally along the antero-posterior axis in the central canal of zebrafish embryos. In the cfap298tm304 mutant, reduction of cilia motility slows transport posteriorly down the central canal and abolishes spontaneous activity of CSF-contacting neurons (CSF-cNs). Loss of the sensory Pkd2l1 channel nearly abolishes CSF-cN calcium activity and single channel opening. Recording from isolated CSFcNs in vitro, we show that CSF-cNs are mechanosensory and require Pkd2l1 to respond to pressure. Additionally, adult pkd2l1 mutant zebrafish develop an exaggerated spine curvature, reminiscent of kyphosis in humans. These results indicate that CSF-cNs are mechanosensory cells whose Pkd2l1-driven spontaneous activity reflects CSF flow in vivo. Furthermore, Pkd2l1 in CSF-cNs contributes to maintenance of natural curvature of the spine.
掲載誌名
Nature Communications
ISSN
20411723
cat書誌ID
AA12645905
出版者
Springer Nature
9
開始ページ
3804
発行日
2018-09-18
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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出版社版DOI
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言語
eng
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出版社版
部局
医学系