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ID 114181
著者
Utami, Trianna W. The University of Tokushima
Hagita, Hiroko The University of Tokushima
Yanuaryska, Ryna D. The University of Tokushima
キーワード
DNA methylation
epigenetic
regulation
gain-of-function
histone modification
Sp6
資料タイプ
学術雑誌論文
抄録
To investigate the function of specificity protein 6 (SP6) transcription factor by gain-of-function procedure, we established cytomegalovirus (CMV) promoter-driven Sp6 stable transformants, C9 cells, using dental epithelial-derived cells. Initially, C9 cells produced a significant amount of SP6 protein. However, SP6 expression was reduced in these cells upon long-term culture. We could detect Sp6 transcripts in C9 cells by RT-PCR throughout the passages, although the CMV promoter is known to be epigenetically silenced. We recently found that SP6 was a short-lived protein that was degraded by a ubiquitin-independent proteasome pathway, although it is yet unclear how Sp6 expression was regulated during culture. Thus, we studied the possibility of epigenetic regulation of Sp6 expression. Comparative analysis of endogenous and exogenous Sp6 mRNA expressions demonstrated the specific down-regulation of exogenous Sp6 mRNA levels during culture passages. A DNA methyltransferase inhibitor, 5-Aza-2'-deoxycytidine (5AC), and a histone deacetylase inhibitor, valproic acid (VPA), enhanced or induced SP6 protein expression up to passage 28 without enhancing the mRNA level. The dramatic up-regulation of exogenous Sp6 mRNA was uniquely observed only at passage 50 by 5AC or VPA treatment. These findings indicate that multiple epigenetic regulatory mechanisms operate to fine-tune Sp6 expression during long-term culture.
掲載誌名
The Indonesian Journal of Dental Research
ISSN
20878710
24423300
出版者
Universitas Gadjah Mada
1
3
開始ページ
134
終了ページ
142
発行日
2011
権利情報
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License(https://creativecommons.org/licenses/by-sa/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系