アクセス数 : ?
ダウンロード数 : ?
ID 114216
タイトル別表記
Stat5 determines the development of mammary alveolar cells
著者
三好, 圭子 National Institutes of Health|The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Shillingford, Jonathan M. National Institutes of Health
Smith, Gilbert H. National Institutes of Health
Grimm, Sandra L. Baylor College of Medicine
Wagner, Kay-Uwe National Institutes of Health|University of Nebraska Medical Center
Oka, Takami National Institutes of Health
Rosen, Jeffrey M. Baylor College of Medicine
Robinson, Gertraud W. National Institutes of Health
Hennighausen, Lothar National Institutes of Health
資料タイプ
学術雑誌論文
抄録
Functional development of mammary epithelium during pregnancy depends on prolactin signaling. However, the underlying molecular and cellular events are not fully understood. We examined the specific contributions of the prolactin receptor (PrlR) and the signal transducers and activators of transcription 5a and 5b (referred to as Stat5) in the formation and differentiation of mammary alveolar epithelium. PrlR- and Stat5-null mammary epithelia were transplanted into wild-type hosts, and pregnancy-mediated development was investigated at a histological and molecular level. Stat5-null mammary epithelium developed ducts but failed to form alveoli, and no milk protein gene expression was observed. In contrast, PrlR-null epithelium formed alveoli-like structures with small open lumina. Electron microscopy revealed undifferentiated features of organelles and a perturbation of cell–cell contacts in PrlR- and Stat5-null epithelia. Expression of NKCC1, an Na-K-Cl cotransporter characteristic for ductal epithelia, and ZO-1, a protein associated with tight junction, were maintained in the alveoli-like structures of PrlR- and Stat5-null epithelia. In contrast, the Na-Pi cotransporter Npt2b, and the gap junction component connexin 32, usually expressed in secretory epithelia, were undetectable in PrlR- and Stat5- null mice. These data demonstrate that signaling via the PrlR and Stat5 is critical for the proliferation and differentiation of mammary alveoli during pregnancy.
掲載誌名
Journal of Cell Biology
ISSN
00219525
15408140
cat書誌ID
AA00694812
AA1203766X
出版者
Rockefeller University Press
155
4
開始ページ
531
終了ページ
542
発行日
2001-11-12
権利情報
© 2001 Keiko Miyoshi et al. Originally published in Journal of Cell Biology. https://doi.org/10.1083/jcb.200107065
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系