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ID 114562
タイトル別表記
JRAB shifts “dancing style” of cell clusters
著者
Yoshizawa, Shin RIKEN
Nishimura, Masaomi RIKEN
Tsuchiya, Yuko National Institutes of Biomedical Innovation, Health and Nutrition|Osaka University
Matsushita, Natsuki Ehime University|Aichi Medical University
Mizuguchi, Chiharu Tokushima University
斎藤, 博幸 Tokushima University|Kyoto Pharmaceutical University KAKEN研究者をさがす
Ueno, Takato Hokkaido University
Matsushita, Sachi Ehime University
Haga, Hisashi Hokkaido University
Deguchi, Shinji Nagoya Institute of Technology|Osaka University
Mizuguchi, Kenji National Institutes of Biomedical Innovation, Health and Nutrition
Yokota, Hideo RIKEN
資料タイプ
学術雑誌論文
抄録
In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein of Rab13 GTPase, provides the “law and order” allowing myriad cells to behave as a single unit just by changing its conformation. First, we generated a structural model of JRAB/MICAL-L2 by a combination of bioinformatic and biochemical analyses and showed how JRAB/MICAL-L2 interacts with Rab13 and how its conformational change occurs. We combined cell biology, live imaging, computational biology, and biomechanics to show that impairment of conformational plasticity in JRAB/MICAL-L2 causes excessive rigidity and loss of directionality, leading to imbalance in cell group behavior. This multidisciplinary approach supports the concept that the conformational plasticity of a single molecule provides “law and order” in collective cell migration.
掲載誌名
Molecular Biology of the Cell
ISSN
19394586
出版者
The American Society for Cell Biology
27
20
開始ページ
3095
終了ページ
3108
発行日
2016-08-31
権利情報
© 2016 Sakane et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
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出版社版DOI
出版社版URL
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言語
eng
著者版フラグ
出版社版
部局
医学系
薬学系