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ID 114610
タイトル別表記
分子内結合が調節するJRABのLIMドメインによるアクチン細胞骨格の再編成
著者
三宅, 一央 徳島大学大学院医科学教育部(医学専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Tsuchiya, Yuko The National Institute of Advanced Industrial Science and Technology|National Institutes of Biomedical Innovation, Health and Nutrition
Tomida, Yoko Tokushima University
井本, 逸勢 Aichi Cancer Center Research Institute|Nagoya University KAKEN研究者をさがす
Watanabe, Shio Thermo Fisher Scientific
Higo, Daisuke Thermo Fisher Scientific
Mizuguchi, Kenji National Institutes of Biomedical Innovation, Health and Nutrition
キーワード
JRAB/MICAL-L2
Actin Cytoskeleton
Intramolecular interaction
LIM domain
資料タイプ
学位論文
抄録
JRAB/MICAL-L2 is an effector protein of Rab13, a member of the Rab family of small GTPase. JRAB/MICAL-L2 consists of a calponin homology domain, a LIM domain, and a coiled-coil domain. JRAB/MICAL-L2 engages in intramolecular interaction between the N-terminal LIM domain and the C-terminal coiled-coil domain, and changes its conformation from closed to open under the effect of Rab13. Open-form JRAB/MICAL-L2 induces the formation of peripheral ruffles via an interaction between its calponin homology domain and filamin. Here, we report that the LIM domain, independent of the C-terminus, is also necessary for the function of open-form JRAB/MICAL-L2. In mechanistic terms, two zinc finger domains within the LIM domain bind the first and second molecules of actin at the minus end, potentially inhibiting the depolymerization of actin filaments (F-actin). The first zinc finger domain also contributes to the intramolecular interaction of JRAB/MICAL-L2. Moreover, the residues of the first zinc finger domain that are responsible for the intramolecular interaction are also involved in the association with F-actin. Together, our findings show that the function of open-form JRAB/MICAL-L2 mediated by the LIM domain is fine-tuned by the intramolecular interaction between the first zinc finger domain and the C-terminal domain.
掲載誌名
Scientific Reports
ISSN
20452322
出版者
Springer Nature
9
開始ページ
12794
発行日
2019-09-05
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kazuhisa Miyakeの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3359号
学位記番号
甲医第1442号
学位授与年月日
2020-02-27
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
技術支援部
薬学系