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ID 114673
タイトル別表記
ショウガオールはヒト歯肉線維芽細胞において酸化ストレス反応の調節を介してAGEs誘導性のIL-6およびICAM-1産生を抑制する
著者
野中, 康平 徳島大学大学院口腔科学教育部(口腔科学専攻)
キーワード
advanced glycation end-products
6-shogaol
ROS
antioxidant enzyme
IL-6
ICAM-1
human gingival fibroblasts
資料タイプ
学位論文
抄録
Advanced glycation end-products (AGEs) cause diabetes mellitus (DM) complications and accumulate more highly in periodontal tissues of patients with periodontitis and DM. AGEs aggravate periodontitis with DM by increasing the expression of inflammation-related factors in periodontal tissues. 6-Shogaol, a major compound in ginger, has anti-inflammatory and anti-oxidative activities. However, the influence of shogaol on DM-associated periodontitis is not well known. In this study, the effects of 6-shogaol on AGEs-induced oxidative and anti-oxidative responses, and IL-6 and ICAM-1 expression in human gingival fibroblasts (HGFs) were investigated. When HGFs were cultured with 6-shogaol and AGEs, the activities of reactive oxygen species (ROS) and antioxidant enzymes (heme oxygenase-1 [HO-1] and NAD(P)H quinone dehydrogenase 1 [NQO1]), and IL-6 and ICAM-1 expressions were investigated. RAGE expression and phosphorylation of MAPKs and NF-κB were examined by western blotting. 6-Shogaol significantly inhibited AGEs-induced ROS activity, and increased HO-1 and NQO1 levels compared with the AGEs-treated cells. The AGEs-stimulated expression levels of receptor of AGE (RAGE), IL-6 and ICAM-1 and the phosphorylation of p38, ERK and p65 were attenuated by 6-shogaol. These results suggested that 6-shogaol inhibits AGEs-induced inflammatory responses by regulating oxidative and anti-oxidative activities and may have protective effects on periodontitis with DM.
掲載誌名
Molecules
ISSN
14203049
出版者
MDPI
24
20
開始ページ
3705
発行日
2019-10-15
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kohei Nonakaの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3377号
学位記番号
甲口第456号
学位授与年月日
2020-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
病院
歯学系