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ID 114676
タイトル別表記
Cystic Fibrosis Transmembrane Conductance Regulatorは微小管依存的なカンピロバクター・ジェジュニの侵入を抑制する
Role of CFTR in Campylobacter jejuni infection
著者
木戸, 純子 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Amano, Sachie Tokushima University
Hatayama, Sho Tokushima University
Nguyen, Quoc Anh Tokushima University
Sato, Yuri Tokushima University
Kanda, Yuna Tokushima University
Tentaku, Aya Tokushima University
Fukushima, Shiho Tokushima University
キーワード
CFTR
Campylobacter jejuni
cell invasion
host response to infection
intracellular bacteria
microtubule
資料タイプ
学位論文
抄録
Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasΔF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.
Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection.
掲載誌名
Infection and Immunity
ISSN
10985522
cat書誌ID
AA12050872
AA1200309X
出版者
American Society for Microbiology
85
10
開始ページ
e00311-17
発行日
2017-09-20
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Junko Kidoの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2017 American Society for Microbiology
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3409号
学位記番号
甲栄第270号
学位授与年月日
2020-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系
生物資源系