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ID 114680
タイトル別表記
非微生物素材表面への大腸菌の初期付着を抑制する穀類抽出液の分析
著者
酒井, 仁美 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Badr, Hoida Ali Badr Tokushima University
キーワード
Cereals
Escherichia coli
Attachment
Abiotic materials
資料タイプ
学位論文
抄録
We examined the initial attachment of E. coli to abiotic surfaces conditioned with cereal extracts. The extracts were water-soluble fractions prepared from flours of barley, quinoa, rice and wheat. Strains used were E. coli ATCC 8739, E. coli NBRC 3301, E. coli NBRC 3302, E. coli NBRC 13168, E. coli NBRC 13891, and E. coli O157:H7 sakai. When surfaces of glass and stainless steel were conditioned at 25°C for 30 min with 0.5% cereal extracts, significantly lower numbers of E. coli cells attached to the conditioned surfaces than unconditioned ones, irrespective of strains used. The highest activity in reduction of the number of E. coli cells attached to the abiotic surfaces was found in the wheat extract. The suppressive activity was stable after treatments of the extract by autoclave and enzymatic digestion with α-amylase and Proteinase K. We purified the active compound by ammonium sulfate fractionation and gel filtration with HiPrep 16/60 Sephacryl S-200 HR after the enzymatic treatments. The purified compound showed an average molecular mass of about 300 kDa by light-scattering measurements. Analyses of its components indicated that the active compound was arabinoxylan; the molar ratios were 1.0 (arabinose) to 2.46 (xylose). Commercially available arabinoxylan (average molecular mass: 370 kDa) also showed the similar activity. To our knowledge, this is the first report on a dietary fiber from cereals which suppresses the initial attachment of E. coli to abiotic surfaces.
掲載誌名
European Food Research and Technology
ISSN
14382377
14382385
出版者
Springer
246
5
開始ページ
947
終了ページ
953
発行日
2020-03-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Hitomi Sakaiの学位論文として提出され,学位審査・授与の対象となっている。
論文本文は2021-03-02以降公開予定
This is a post-peer-review, pre-copyedit version of an article published in European Food Research and Technology. The final authenticated version is available online at: https://doi.org/10.1007/s00217-020-03444-9
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
jpn
著者版フラグ
その他
文科省報告番号
甲第3411号
学位記番号
甲栄第272号
学位授与年月日
2020-03-12
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
技術支援部
医学系
生物資源系