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ID 115027
タイトル別表記
Evaluation of the histological and mechanical features of tendon healing in a rabbit model
著者
キーワード
Tendon
Collagen
Healing
Second-harmonic-generation microscopy
Tensile testing
資料タイプ
学術雑誌論文
抄録
Objectives
This study aimed to evaluate the histological and mechanical features of tendon healing in a rabbit model with second-harmonic-generation (SHG) imaging and tensile testing.
Materials and Methods
A total of eight male Japanese white rabbits were used for this study. The flexor digitorum tendons in their right leg were sharply transected, and then were repaired by intratendinous stitching. At four weeks post-operatively, the rabbits were killed and the flexor digitorum tendons in both right and left legs were excised and used as specimens for tendon healing (n = 8) and control (n = 8), respectively. Each specimen was examined by SHG imaging, followed by tensile testing, and the results of the two testing modalities were assessed for correlation.
Results
While the SHG light intensity of the healing tendon samples was significantly lower than that of the uninjured tendon samples, 2D Fourier transform SHG images showed a clear difference in collagen fibre structure between the uninjured and the healing samples, and among the healing samples. The mean intensity of the SHG image showed a moderate correlation (R2 = 0.37) with Young’s modulus obtained from the tensile testing.
Conclusion
Our results indicate that SHG microscopy may be a potential indicator of tendon healing.
掲載誌名
Bone & Joint Research
ISSN
20463758
出版者
The British Editorial Society of Bone & Joint Surgery
5
11
開始ページ
577
終了ページ
585
発行日
2016-11-23
権利情報
© 2016 Yasui et al. This is an open-access article distributed under the terms of the Creative Commons Attributions licence (CC-BY-NC)(https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
ポストLEDフォトニクス研究所
理工学系
病院