ID | 115112 |
タイトル別表記 | Cerebral Glycogen Distribution and Aging
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著者 |
Oe, Yuki
RIKEN Brain Science Institute
Ashida, Hitoshi
Kobe University
Nakamura, Kouichi C.
Kyoto University
Hirase, Hajime
RIKEN Brain Science Institute|Saitama University
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キーワード | astrocytes
focused microwave irradiation
glycogen
immunohistochemistry
aging
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資料タイプ |
学術雑誌論文
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抄録 | In the brain, glycogen metabolism has been implied in synaptic plasticity and learning, yet the distribution of this molecule has not been fully described. We investigated cerebral glycogen of the mouse by immunohistochemistry (IHC) using two monoclonal antibodies that have different affinities depending on the glycogen size. The use of focused microwave irradiation yielded well‐defined glycogen immunoreactive signals compared with the conventional periodic acid‐Schiff method. The IHC signals displayed a punctate distribution localized predominantly in astrocytic processes. Glycogen immunoreactivity (IR) was high in the hippocampus, striatum, cortex, and cerebellar molecular layer, whereas it was low in the white matter and most of the subcortical structures. Additionally, glycogen distribution in the hippocampal CA3‐CA1 and striatum had a ‘patchy’ appearance with glycogen‐rich and glycogen‐poor astrocytes appearing in alternation. The glycogen patches were more evident with large‐molecule glycogen in young adult mice but they were hardly observable in aged mice (1–2 years old). Our results reveal brain region‐dependent glycogen accumulation and possibly metabolic heterogeneity of astrocytes.
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掲載誌名 |
GLIA
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ISSN | 10981136
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cat書誌ID | AA10687234
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出版者 | Wiley
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巻 | 64
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号 | 9
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開始ページ | 1532
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終了ページ | 1545
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発行日 | 2016-06-29
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権利情報 | This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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言語 |
eng
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出版社版
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部局 |
歯学系
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