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ID 115291
タイトル別表記
歯髄幹細胞からの分泌因子は、顎関節変形性関節症の治療に多面的な効果を示す
著者
小笠原, 直子 徳島大学大学院口腔科学教育部(口腔科学専攻)
Liu, Yao Tokushima University
Xia, L. Tokushima University
Sakamaki, T. Tokushima University
Hibi, H. Nagoya University
キーワード
Dental pulp stem cell
Conditioned medium
Chondrocyte
Cartilage regeneration
Temporomandibular joint
Osteoarthritis
資料タイプ
学位論文
抄録
Objective: Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease characterized by progressive cartilage degeneration, abnormal bone remodeling, and chronic pain. In this study, we aimed to investigate effective therapies to reverse or suppress TMJOA progression.
Design: To this end, we performed intravenous administration of serum free conditioned media from human exfoliated deciduous teeth stem cells (SHED-CM) into a mechanical-stress induced murine TMJOA model.
Results: SHED-CM administration markedly suppressed temporal muscle inflammation, and improved bone integrity and surface smoothness of the destroyed condylar cartilage. Moreover, SHED-CM treatment decreased the number of IL-1β, iNOS, and MMP-13 expressing chondrocytes, whereas it specifically increased PCNA-positive cells in the multipotent polymorphic cell layer. Notably, the numbers of TdT-mediated dUTP nick end labeling (TUNEL)-positive apoptotic chondrocytes in the SHED-CM treated condyles were significantly lower than in those treated with DMEM, whereas the proteoglycan positive area was restored to a level similar to that of the sham treated group, demonstrating that SHED-CM treatment regenerated the mechanical-stress injured condylar cartilage and subchondral bone. Secretome analysis revealed that SHED-CM contained multiple therapeutic factors that act in osteochondral regeneration.
Conclusions: Our data demonstrated that SHED-CM treatment promoted the regeneration and repair of mechanical-stress induced mouse TMJOA. Our observations suggest that SHED-CM has potential to be a potent tissue-regenerating therapeutic agent for patients with severe TMJOA.
掲載誌名
Osteoarthritis and Cartilage
ISSN
10634584
cat書誌ID
AA10998176
AA11537860
出版者
Osteoarthritis Research Society International|Elsevier Ltd.
28
6
開始ページ
831
終了ページ
841
発行日
2020-04-06
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者N. Ogasawaraの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3444号
学位記番号
甲口第465号
学位授与年月日
2020-09-10
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系