ID | 115493 |
著者 |
Demetriadou, Anthi
The Cyprus Institute of Neurology and Genetics|The Cyprus School of Molecular Medicine
Morales-Sanfrutos, Julia
Imperial College London
Nearchou, Marianna
The Cyprus Institute of Neurology and Genetics
Kyriacou, Kyriacos
The Cyprus School of Molecular Medicine|The Cyprus Institute of Neurology and Genetics
Tate, Edward W.
Imperial College London
Drousiotou, Anthi
The Cyprus Institute of Neurology and Genetics|The Cyprus School of Molecular Medicine
Petrou, Petros P.
The Cyprus Institute of Neurology and Genetics|The Cyprus School of Molecular Medicine
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キーワード | Stbd1
Glycogen
Endoplasmic reticulum
Mitochondria
N-myristoylation
Mitochondria-associated membranes
Organized smooth endoplasmic reticulum
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資料タイプ |
学術雑誌論文
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抄録 | Starch binding domain-containing protein 1 (Stbd1) is a carbohydrate-binding protein that has been proposed to be a selective autophagy receptor for glycogen. Here, we show that mouse Stbd1 is a transmembrane endoplasmic reticulum (ER)-resident protein with the capacity to induce the formation of organized ER structures in HeLa cells. In addition to bulk ER, Stbd1 was found to localize to mitochondria-associated membranes (MAMs), which represent regions of close apposition between the ER and mitochondria. We demonstrate that N-myristoylation and binding of Stbd1 to glycogen act as major determinants of its subcellular targeting. Moreover, overexpression of non-myristoylated Stbd1 enhanced the association between ER and mitochondria, and further induced prominent mitochondrial fragmentation and clustering. Conversely, shRNA-mediated Stbd1 silencing resulted in an increase in the spacing between ER and mitochondria, and an altered morphology of the mitochondrial network, suggesting elevated fusion and interconnectivity of mitochondria. Our data unravel the molecular mechanism underlying Stbd1 subcellular targeting, support and expand its proposed function as a selective autophagy receptor for glycogen and uncover a new role for the protein in the physical association between ER and mitochondria.
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掲載誌名 |
Journal of Cell Science
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ISSN | 00219533
14779137
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cat書誌ID | AA00694823
AA11720335
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出版者 | The Company of Biologists
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巻 | 130
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号 | 5
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開始ページ | 903
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終了ページ | 915
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発行日 | 2017-03-01
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権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
歯学系
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