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ID 115930
タイトル別表記
Pex14p phosphorylation regulates peroxisome import
著者
Yamashita, Koichiro Kyushu University
Tamura, Shigehiko Kyushu University
Honsho, Masanori Kyushu University|Institute of Rheological Functions of Food
Yada, Hiroto Kyushu University
Yagita, Yuichi Kyushu University
Fujiki, Yukio Kyushu University|Institute of Rheological Functions of Food
資料タイプ
学術雑誌論文
抄録
Peroxisomal matrix proteins are imported into peroxisomes via membrane-bound docking/translocation machinery. One central component of this machinery is Pex14p, a peroxisomal membrane protein involved in the docking of Pex5p, the receptor for peroxisome targeting signal type 1 (PTS1). Studies in several yeast species have shown that Pex14p is phosphorylated in vivo, whereas no function has been assigned to Pex14p phosphorylation in yeast and mammalian cells. Here, we investigated peroxisomal protein import and its dynamics in mitotic mammalian cells. In mitotically arrested cells, Pex14p is phosphorylated at Ser-232, resulting in a lower import efficiency of catalase, but not the majority of proteins including canonical PTS1 proteins. Conformational change induced by the mitotic phosphorylation of Pex14p more likely increases homomeric interacting affinity and suppresses topological change of its N-terminal part, thereby giving rise to the retardation of Pex5p export in mitotic cells. Taken together, these data show that mitotic phosphorylation of Pex14p and consequent suppression of catalase import are a mechanism of protecting DNA upon nuclear envelope breakdown at mitosis.
掲載誌名
Journal of Cell Biology
ISSN
00219525
15408140
cat書誌ID
AA00694812
AA1203766X
出版者
Rockefeller University Press
219
10
開始ページ
e202001003
発行日
2020-08-26
権利情報
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
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言語
eng
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部局
先端酵素学研究所