ID | 116525 |
タイトル別表記 | SlIAA9 Controls Tomato Elongation
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著者 |
Abe-Hara, Chihiro
Tokushima University
Ueta, Risa
Tokushima University
Hashimoto, Ryosuke
Tokushima University
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キーワード | parthenocarpy
photomorphogenesis
elongation growth
shoot growth
auxin
SlIAA9
CRISPR-Cas9
tomato
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資料タイプ |
学術雑誌論文
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抄録 | Tomato INDOLE-3-ACETIC ACID9 (SlIAA9) is a transcriptional repressor in auxin signal transduction, and SlIAA9 knockout tomato plants develop parthenocarpic fruits without fertilization. We generated sliaa9 mutants with parthenocarpy in several commercial tomato cultivars (Moneymaker, Rio Grande, and Ailsa Craig) using CRISPR-Cas9, and null-segregant lines in the T1 generation were isolated by self-pollination, which was confirmed by PCR and Southern blot analysis. We then estimated shoot growth phenotypes of the mutant plants under different light (low and normal) conditions. The shoot length of sliaa9 plants in Moneymaker and Rio Grande was smaller than those of wild-type cultivars in low light conditions, whereas there was not clear difference between the mutant of Ailsa Craig and the wild-type under both light conditions. Furthermore, young seedlings in Rio Grande exhibited shade avoidance response in hypocotyl growth, in which the hypocotyl lengths were increased in low light conditions, and sliaa9 mutant seedlings of Ailsa Craig exhibited enhanced responses in this phenotype. Fruit production and growth rates were similar among the sliaa9 mutant tomato cultivars. These results suggest that control mechanisms involved in the interaction of AUX/IAA9 and lights condition in elongation growth differ among commercial tomato cultivars.
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掲載誌名 |
Frontiers in Plant Science
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ISSN | 1664462X
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出版者 | Frontiers Media S.A.
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巻 | 12
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開始ページ | 627832
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発行日 | 2021-05-20
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権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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言語 |
eng
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部局 |
生物資源系
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