ID | 116915 |
タイトル別表記 | Archaea ATP-Dependent RNA Ligase
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著者 |
Liu, Yancheng
University of Tsukuba
Takagi, Yuko
National Institute of Advanced Industrial Science and Technology
Sugijanto, Milyadi
University of Tsukuba
Duong My Nguyen, Kieu
University of Tsukuba
Hori, Hiroyuki
Ehime University
Ho, C. Kiong
University of Tsukuba
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キーワード | circular RNA
RNA ligase
thermococcus kodakarensis KOD1
rRNA processing
C/D box sRNAs
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資料タイプ |
学術雑誌論文
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抄録 | RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this study, we generated an allelic knockout of ATP-dependent RNA ligase (Rnl) in the hyperthermophilic archaeon Thermococcus kodakarensis to identify its biological targets. A comparative analysis of circular RNA reveals that the Rnl-knockout strain represses circularization of C/D box sRNAs without affecting the circularization of tRNA and rRNA processing intermediates. Recombinant archaeal Rnl could circularize C/D box sRNAs with a mutation in the conserved C/D box sequence element but not when the terminal stem structures were disrupted, suggesting that proximity of the two ends could be critical for intramolecular ligation. Furthermore, T. kodakarensis accumulates aberrant RNA fragments derived from ribosomal RNA in the absence of Rnl. These results suggest that Rnl is responsible for C/D box sRNA circularization and may also play a role in ribosomal RNA processing.
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掲載誌名 |
Frontiers in Molecular Biosciences
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ISSN | 2296889X
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出版者 | Frontiers Media S.A.
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巻 | 9
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開始ページ | 811548
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発行日 | 2022-03-25
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権利情報 | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
理工学系
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