直近一年間の累計
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ID 117466
著者
Murakawa, Naomi Tokushima University
Kanoh, Mizuho Tokushima University
Park, Si-Bum Kyoto University
Kishino, Shigenobu Kyoto University
Ogawa, Jun Kyoto University
キーワード
Fusarium solani
10-Hydroxyoctadecanoic acid
10-Hydroxy-cis-12-octadecenoic acid
10-Oxo-octadecanoic acid
Fatty acid hydration
Crude glycerol
資料タイプ
学術雑誌論文
抄録
Strain D2 was isolated from a natural sample as a crude glycerol-assimilating microorganism. The ITS-5.8S rDNA sequence of strain D2 was most similar to that of Fusarium solani deposited in the NCBI database. Strain D2 accumulated 10-hydroxy-cis-12-octadecenoic acid (HYA), 10-hydroxyoctadecanoic acid (HYB), and 10-oxooctadecanoic acid (KetoB) in a medium containing crude glycerol, as a carbon source, and yeast extract, named CG medium. The growth and HYB production of strain D2 depended on the crude glycerol concentration in the medium. Strain D2 produced 2.20 g/L (40% of total fatty acids) of HYB on cultivation in CG medium containing 8% crude glycerol. When strain D2 was cultivated in CG medium containing 6% crude glycerol, the yield of HYB on cultivation under 4 days-shaking and 3 days-static conditions reached 1.19 g/L, which was 2.2 times higher than that under 7 days-shaking conditions and accounted for 53% of total fatty acids. The fungus was found not only to efficiently produce fatty acids utilizing crude glycerol, but also to be the first filamentous fungus to produce hydroxy and oxo fatty acids such as HYB, HYA, and KetoB.
掲載誌名
Biocatalysis and Agricultural Biotechnology
ISSN
18788181
出版者
Elsevier
39
開始ページ
102286
発行日
2022-01-17
権利情報
© 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系