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ID 118437
タイトル別表記
Detection Chip for Malaria
著者
Yatsushiro, Shouki National Institute of Advanced Industrial Science and Technology
Yamamura, Shohei National Institute of Advanced Industrial Science and Technology
山口, 裕加 National Institute of Advanced Industrial Science and Technology 徳島大学 教育研究者総覧
Tamiya, Eiichi Osaka University
Horii, Toshihiro Osaka University
馬場, 嘉信 National Institute of Advanced Industrial Science and Technology|Nagoya University
片岡, 正俊 National Institute of Advanced Industrial Science and Technology
資料タイプ
学術雑誌論文
抄録
Background: Malaria is one of the major human infectious diseases in many endemic countries. For prevention of the spread of malaria, it is necessary to develop an early, sensitive, accurate and conventional diagnosis system.
Methods and Findings: A cell microarray chip was used to detect for malaria-infected erythrocytes. The chip, with 20,944 microchambers (105 µm width and 50 µm depth), was made from polystyrene, and the formation of monolayers of erythrocytes in the microchambers was observed. Cultured Plasmodium falciparum strain 3D7 was used to examine the potential of the cell microarray chip for malaria diagnosis. An erythrocyte suspension in a nuclear staining dye, SYTO 59, was dispersed on the chip surface, followed by 10 min standing to allow the erythrocytes to settle down into the microchambers. About 130 erythrocytes were accommodated in each microchamber, there being over 2,700,000 erythrocytes in total on a chip. A microarray scanner was employed to detect any fluorescence-positive erythrocytes within 5 min, and 0.0001% parasitemia could be detected. To examine the contamination by leukocytes of purified erythrocytes from human blood, 20 µl of whole blood was mixed with 10 ml of RPMI 1640, and the mixture was passed through a leukocyte isolation filter. The eluted portion was centrifuged at 1,000×g for 2 min, and the pellet was dispersed in 1.0 ml of medium. SYTO 59 was added to the erythrocyte suspension, followed by analysis on a cell microarray chip. Similar accommodation of cells in the microchambers was observed. The number of contaminating leukocytes was less than 1 on a cell microarray chip.
Conclusion: The potential of the cell microarray chip for the detection of malaria-infected erythrocytes was shown, it offering 10–100 times higher sensitivity than that of conventional light microscopy and easy operation in 15 min with purified erythrocytes.
掲載誌名
PLOS ONE
ISSN
19326203
出版者
PLOS
5
10
開始ページ
e13179
発行日
2010-10-13
権利情報
© 2010 Yatsushiro et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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言語
eng
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先端酵素学研究所